Single-molecule detection and identification via DNA nanotechnology

  • June 16, 2021, 4:00 pm US/Central
  • William Shih, Harvard
  • Chris Stoughton

Fermilab employees and users can access the Zoom link below (Services login required):

When: Wednesday, June 16, 2021 at 4:00 PM – 5:00 PM.

Location: ZOOM ONLY

Please note: you will need the passcode to enter the zoom

Anyone else can obtain the Zoom link the day of the colloquium by emailing Barb Kronkow at


DNA origami, in which a long scaffold strand is assembled with a large number of short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication of shapes up to 100 nm in size. However, the scaffold represents about half the mass of an origami, therefore the origami size is restricted by the length of the scaffold. Furthermore, it is impractical and prohibitively expensive to scale the length of the scaffold. Here I will discuss a strategy, that we call crisscross ultracooperative assembly, that combines all-or-nothing scaffold-dependent initiation of folding with scaffold-independent growth, therefore allowing for sizes unbounded by the length of the scaffold. A major application will be digital counting of molecular analytes, where each molecular detection event triggers growth of a single filament resolvable by low-cost microscopy.